Preparation of Monoclonal Anti-human PDGF Antibodies Using Synthetic Peptide as Immunogen
نویسندگان
چکیده
منابع مشابه
Production and characterization of monoclonal antibodies to the c-erbB-2 proto-oncogene protein using a synthetic peptide immunogen.
NADIA L. TUZI,* DEON J. VENTER,? JACKIE A. REIDLINGER,$ CHRISTOPHER J. DEAN$ and WILLIAM J. GULLICK* *Imperial Cancer Research Fund Oncology Group, M. R. C. Cyclotron Building, Hummersmith Hospital, Du Cane Road, London WI2 OHS, U .K . , tLudwig Institute for Cancer Research, 91 Riding House Street, London WIP 8BT, U.K. and $Institute of Cancer Research, Section of Medicine, CIifOn Avenue, Belm...
متن کاملSecond generation anti-MUC1 peptide monoclonal antibodies.
Second generation antibodies to mammary mucins were produced by immunizing mice with a peptide with a sequence deduced from that of the MUC1 complementary DNA sequence (PAHGVTSAPDTRPAPGSTAP). Four monoclonal antibodies (BCP7-10) were produced which gave different reactions. BCP8 was similar in tissue reactivity (by immunoperoxidase staining) to anti-breast cancer or anti-human milk fat globule ...
متن کاملHuman nesidioblastosis tissue as an immunogen for generation of islet cell specific monoclonal antibodies.
Nesidioblastosis pancreas was used as an immunogen in BALB/c-mice to generate monoclonal antibodies against structures of human islet cells. Seven clones were selected by screening 311 growing hybridomas for reactivity with the rat insulinoma cell line, RIN m5F, and with cryostat sections from human pancreas. None of the selected clones reacted with pancreatic hormones or endocrine-specific pep...
متن کاملMeasurement of Affinity Constant of Anti-human IgG Monoclonal Antibodies by an ELISA-based Method
Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...
متن کاملMONOCLONAL ANTIBODIES TO HUMAN ALPHA-FETOPROTEIN
Alpha-fetoprotein (AFP), a serum glycoprotein belonging to the onco-developmental proteins group, serves as a marker both in cancer research and in studies concerning fetal development and fetal pathophysiology. Monoclonal anti-AFP antibodies are essential reagents in developing appropriate techniques for measurement of this protein. In this study, in order to produce anti-AFP monoclonal a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Japan Atherosclerosis Society
سال: 1988
ISSN: 0386-2682,2185-8284
DOI: 10.5551/jat1973.16.1_37